Virology Discussion

Virology Discussion

Viruses are not life-forms themselves, because they can’t survive on their own. They must take over about the biology of viruses. Viruses are very small particles that act as cellular parasites. It’s cellular machinery in order to survive.
This chapter initially talked about factors common to all viruses, and then turned to those aspects that are particular to HIV. HIV is a member of a class of virus know as retroviruses. While most viruses use DNA as their genetic material, retroviruses use RNA Reverse transcriptase is a protein in retroviruses that copies RNA into DNA, and then helps the viral DNA integrate into the cellular chromosomes, where it will remain for the life of that infected cell.
For this discussion, choose one example of the viral biology to focus on. Choose one aspect that was unusual interesting to you. Do some research to find out a little more about it. Here is a list of examples, but you can think of other Ones as well:
a. the retrovirus life cycle
b. anti-HIV therapies
c. the ELISA “HIV test”
d. the fate of HIV infected cells
e. HIV latency
f. direct cell to cell spread of the HIV
g. how HIV kills T-cells that aren’t infected with HIV
h. If viruses aren’t alive, what motivates them to keep going on?
Each student should write up one primary post of about 150-200 words.

My Right to Die

MLA FORMAT
180 WORD COUNT MIN.
PROPER IN TEXT CIATION
QUESTION 1,3 and 4 NEED 2 ACADEMIC RESOURCES TO SUPPORT YOUR ANSWER
**Read Case Study Attached & Answer Question 1**
One of the most important aspects of nursing is ensuring your self-care and moral/ethical well-being when dealing with death and dying situations.  In order to make ethically sound decisions when caring for patients, you must first become intimately familiar with yourself, your personal values and be able to identify when they are in conflict.
Case 9.2:  The Case of Brittany Maynard: “My Right to Die”. 
Question 1) Check in with yourself about what emotions this brings up for you.   Answer question #4:  What is your opinion of Brittany Maynard’s decision to end her life at age 29 years?  Please explore your rationale and explain and find a source to support your answer.
Question 1.2) Lastly, what part of this course was the most interesting or helpful to you? How do you think what you have learned in this course will impact your future nursing practice and why?
** Read Below & Answer Question 2**
Question 2: In the Discussion Board, explain how the content in Human Anatomy & Physiology will relate to your future career goals as a nurse?
** Read & Answer Question 3**
Question 3: Pick any endocrine disease or disorder. Briefly describe your topic and be sure to include any potential causes, symptoms or possible treatments. You should have a minimum of 2 academically appropriate resources.
** Read & Answer Question 4*
Question 4: Please share a time during the past year when you had to help someone with a nutritional issue, or wish you had been able to help someone. Provide us with a brief description of the situation and the actions you took. What were the outcomes? What techniques did you engage that worked well, and what could you have done differently?

Biopsychology Journal Article

Biopsychology Journal Article

 https://www.nature.com/articles/s41598-017-09744-7
Please use the following link as the main source of the article 
You must write a brief summary of the article and answer the following questions.
The questions you need to answer can be in bullet form 
What is the main focus of the article?
Who is the intended audience?
Why is this article important to biopsychology?
What is your general first impression?
What would you suggest as further research questions?
Where have you noticed the problems addressed in the article in your own life or society? Or How do you think the information in the journal article is a reflection of our society?

Purification of Bovine Brain Tubulins

cell Biology

Purification of Bovine Brain Tubulins
Source: Banerjee, A., M. C. Roach, P. Treka, R. F. Luduena. 1990. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of β-tubulin. J. Biol. Chem. 265: 1794–1799.
Corresponding chapter(s) in the textbook: Chapter 13 (and 4)
Review the following terms before working on the problem: cytoskeleton, microtubules, tubulins, agarose, column chromatography, polyacrylamide gel electrophoresis, Coomassie Blue staining, Western blotting
Read the paper and answer the questions below that refers to the data described in Figure 1 of the paper.
Be prepared to discuss the other experiments described, in class.

Experiment
Tubulin was isolated from bovine brain, purified by phosphocellulose ion exchange chromatography, and further fractionated by affinity chromatography on an anti-2-tubulin-agarose column. (Note: Affinity chromatography is a powerful separation technique based on the specific, noncovalent interaction between two molecules; in this experiment: between β2-tubulin and its antibody. The antibody is immobilized on agarose beads, and the protein solution is passed through the antibody-agarose column. Unbound molecules are washed out with excess sample buffer, and the specifically bound proteins are eluted with a high-salt solution.)
The original tubulin (PC, for phosphocellulose-purified), the unbound fraction (U), and a bound fraction eluted from the column (B) were fractionated by polyacrylamide gel electrophoresis. The gels were subjected to the following procedures:
(a) Coomassie Blue protein staining
(b) Western blot with anti-2-tubulin
(c) Western blot with anti-1-tubulin
Figure

Questions
1. Assess the molar ratio of α- and β-tubulins in the phosphocellulose-purified sample (sample 1). Explain your answer.
2. What conclusion can be drawn from comparing samples 1 and 2?
3. What conclusion can be drawn from comparing samples 1 and 3?
4. Evaluate the specificity of the anti-2-tubulin antibody used in the experiment.
5. Evaluate the specificity of the affinity chromatography step.
© 2016 Sinauer Associates, Inc.
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