Posts

Based on the above stated effects on adenylyl cyclase activity, what type of G-protein is activated by each receptor?

/in /by

You’re studying cellular signaling through G-Protein Coupled Receptors (GPCRs). Specifically you’re working on a pair of newly identified GPCRs, GPCR-A…

You’re studying cellular signaling through G-Protein Coupled Receptors (GPCRs). Specifically you’re working on a pair of newly identified GPCRs, GPCR-A and GPCR-B. Each binds the same small ligand, but activates different heterotrimeric G-proteins that act on adenylyl cyclase.
A (3 points). Your grad student mentor explains that both the receptors and the Gα and Gβγ subunits of the heterotrimeric G-proteins are membrane associated. You’re puzzled, because although you find obvious transmembrane domains in the receptor, you find none in Gα or Gβγ. How are Gα and Gβγ associated with the membrane (be specific)?
B (5 points). You examine the sequences of the seven predicted transmembrane α-helices of GPCR-B. You’re surprised to find that there are hydrophilic amino acid residues in these helices. How can you explain this observation?
You find that the binding of ligand has opposite effects on adenylyl cyclase activity for each receptor. GPCR-A causes an increase in adenylyl cyclase activity, while GPCR-B causes a decrease in adenylyl cyclase activity.
C (2 points). Based on the above stated effects on adenylyl cyclase activity, what type of G-protein is activated by each receptor?
7
Name: ______________________________D (6 points). You have a cell line that expresses both GPCR-A, GPCR-B, the
corresponding G-proteins, and adenylyl cyclase. There is a basal level of adenylyl cyclase activity that produces a baseline cAMP concentration. Your project is to characterize a series of mutations in these components. Will each mutation increase or decrease the intracellular levels of cAMP upon ligand addition (remember, both GPCR-A and GPCR-B bind the same ligand)?
A mutation in GPCR-A that prevents G- protein activation?
A mutation in GPCR-B that prevents G- protein activation?
A mutation in Gs that prevents release of bound GDP.
A mutation in Gi that prevents release of bound GDP.
A mutation in Gs that prevents GTP hydrolysis.
A mutation in Gi that prevents GTP hydrolysis.

Compare and contrast modern and intermediate species adaptations

/in /by

Animal Evolution Paper Due by Email 5/12 2pm

Animal Evolution Paper Due by Email 5/12 2pm
Charles Darwin was one of the most influential scientific figures. For this project, you will create an evolutionary history of a modern day organism. You need to find the organism’s earliest ancestor, and create a timeline of how your organism may have evolved to what it is today. You should also be able to describe certain adaptations your organism acquired and has that allowed and helped its evolution.
Select one of the following organisms:
ElephantBirds
HorseShark
TigerRhinoceros
WhaleGiraffe
CrocodileMan
Dog
Bear
OR you may choose a different organism that you feel strongly about, but please get it approved by me first.
For this paper, you need to use reputable sources (try and stay away from .com websites), and at least three different sources that give you information about your animal. PLEASE CITE THROUGHOUT YOUR PAPER. I will take off several points for improper citations and no works cited/bibliography page.
Format: 4 pages long, double spaced, with a work cited page (5th page). Please use Times New Roman size 12 font, and 1 inch margins on all four sides of the paper. I WILL NOT ACCEPT ANY PAPERS THAT ARE SHORTER THAN FOUR PAGES LONG. This is 10% of your grade and you need to work hard on it- I will not be grading it as easily as the last paper.
Your paper should address the following content (10 points, 10%):
•The earliest ancestor of your organism and the intermediate forms that led to the modern day animal (2 points)
•Compare and contrast modern and intermediate species adaptations (2 points)
•Compare and contrast the environmental conditions that influenced your organisms evolution (i.e. any ice ages etc., 2 points)
•List modern relatives and their adaptations (2 points) i.e. wolves are a modern relative of the dog
•Give a direct timeline of the evolution of your organism’s evolution (2 points) – this can either be a picture that shows all the different organisms your modern day organism evolved from (with dates) or you may write out the timeline in words

Discussions that how expensive is health care of America

/in /by

Differentiation of primary accounts that needed to be considered in the year-end closing process Preparation of the annual healthcare facility by determines the factors which of these will be more sus

Differentiation of primary accounts that needed to be considered in the year-end closing process
Preparation of the annual healthcare facility by determines the factors which of these will be more susceptible to the adjustment by the auditors and a brief explanation of the role of facility management team in reducing the Audit Adjustments
Explanation of Pro forma financial statement and describe how these statements are utilised in determining the proposed project for the healthcare organisation with two examples of healthcare projects that have been analysed by pro forma statement
Discussions that how expensive is health care of America. Briefly explanation of the reason that why the people spend on healthcare in America. With specific examples of the various types of health care spending and amount spending

DOMESTIC VIOLENCE

/in /by

DOMESTIC VIOLENCE- Analyze the impact of intimate partner violence on the individual client.A minimum of 5 appropriate references are used. Nursing journals, Periodicals, Medical journals, Nursing ref

DOMESTIC VIOLENCE-
Analyze the impact of intimate partner violence on the individual client.

  • A minimum of 5 appropriate references are used. Nursing journals, Periodicals, Medical journals, Nursing reference books, governmental web sites, etc. (No sites such as Wikipedia).
  • Proper grammar, punctuation, spelling, sentence structure and word usage. The entire paper shows detail to the topic, and the sentences flow well from one thought to the next.
  • The topic is related to domestic violence identification, prevention, and assisting the victim with recovery.
  • The topic is clearly introduced in the introductory paragraph.
  • The prevalence of the problem is clearly and correctly outlined.
  • Risk factors or preventative measures related to the topic are included.
  • Nursing care and referral to appropriate resources is clearly and correctly outlined.
  • The topic is clearly summarized in the closing paragraph.
  • The paper is 3 – 5 pages (excluding the cover sheet and reference page(s)