Why is Liver broth (covered with a layer of 2% agar ) commonly used as a growth medium, when analysing cans to check for sterility or cause of spoilage?

/in /by

Questions to answer and hand to your demonstrator at the start of week 2 of the can prac class i.e. week 4 of the semester on August 11:
In relationship to Clostridium botulinum spores, what is meant by a 12D heat process?
What types or groups of microorganisms would you expect to find if a can has been under-processed. Explain the reason for your answer.
What is the meaning of leaker spoilage of canned food and how does this occur?
What types of microorganisms would you expect to isolate from a can if there is leaker spoilage?
Suggest two sources of organisms that may contribute to leaker spoilage
What physical tests would you do on the can seam integrity to determine if the can spoilage has been caused by leaker spoilage?
Controls need to be applied to ensure production of a batch of appertised food (in a glass bottle, can or sealed pouch) is shelf stable and safe for consumption. As a brief summary, control of the following seven (7) steps is important to ensure production of appertised (commercialy sterile) shelf-stable cans.
Supply of cans from the can manufacturer
Source, supply and storage of the raw materials
Preparation of the product going into the can
Applying the lids
Retorting the cans
Cooling the cans
Storage and distribution of the cans
Of the steps listed above, state four (4) steps that are the most important to prevent leaker spoilage in a batch of processed cans. Explain why you have chosen each of them.
i.
ii.
iii
iv.
Of the steps listed above, state three steps that are the most important in controlling unerprocessing, in a batch of processed cans. Explain why you have chosen each of them.
i.
ii.
iii
Why is Liver broth (covered with a layer of 2% agar ) commonly used as a growth medium, when analysing cans to check for sterility or cause of spoilage?
Why is Dextrose Tryptone Broth commonly used as a growth medium, when analysing cans to check for sterility or cause of spoilage?
After inoculation of the food into the above media the tubes are incubate at two temperatures i.e. 300C and 550C. Why are both of these temperatures used?

Discuss FGF-7s role in wound healing and relate it to cancer development.

/in /by

critically analyse the effect: over-expression of FGF-7 has in breast and cervical cancer patients. emphasise greatly on cervical cancer patients. discuss changes in cell function such as cell migration, cell proliferation, cell viability. Discuss FGF-7s role in wound healing and relate it to cancer development.
Critically discuss the role of FGF-7 expression in embryonic development; and relate how it may impact or knowledge may be applied/beneficial to cancer research.
critically discuss the associations of over-expression FGF-7 has with cervical cancer and other types of cancer and how it may serve as a novel therapeutic approach for Tyrosine kinase growth factor inhibitors.

Provide comparisons of the invertebrate and vertebrate systems

/in /by

1) Overview and definition of the immune system. The chart on p 486, Fig 24.1A, “An overview of animal immune systems,” is a good visual to use in the overview.
2) Provide comparisons of the invertebrate and vertebrate systems. You will note that all animals have “innate immunity,” but only vertebrates have “acquired/adaptive immunity.”
3) Discuss next the lymphatic organ system, which is the organ system that is involved in BOTH innate and acquired immunity.
4) Address the different types and processes of acquired immunity. This will include: response to specific invaders; lymphocytes (humoral immune response and cell mediated response, including Helper T cells and Cytotoxic T cells); antigens (clonal selection process); and antibodies (weapons of humoral immune response). Not necessarily in this order….HINT: Use the Chapter Review, p. 502, to help you organize this section on acquired immunity.
5) Finally, address health issues, which will include “Disorders of the immune system,” pp 500-501 (autoimmune diseases/examples, immunodeficiency diseases/examples, allergies). Give a list and description of each.1

Outline the nurses role in addressing the top three priority patient health issues using assessment coordination of care and provision of care.

/in /by

Length: 1500 word limit +/- 10% excluding references
Task: This assessment item has 3 parts:
State the case study that you have chosen including whether the assignment addresses part one or two of the case study.
Identify and briefly describe five or more patient health issues from the case study that nurses will address within their scope of practice. You may include risk for or potential for health issues.
Select the three highest priority patient health issues from the ones that have been described. Provide a brief rationale explaining why these patient health issues should be given priority over the other patient health issues that have been described.
2. Rank the three patient health issues that were selected in part 1 in order of priority. Provide a rationale for the order of the rankings. The rationale must be supported with current literature.
3. Outline the nurses role in addressing the top three priority patient health issues using assessment coordination of care and provision of care. Relate the discussion to regulatory frameworks of nursing and health department policies e.g. NMBA competency standards NSW health policy NSQHA standards etc.
Students need to demonstrate their ability to identify and prioritise patient health issues that nurses can address within their scope of practice and rationalise the order of priority. This will enable students to articulate decisions they will need to make in clinical practice. In addition students will be able to demonstrate their ability to write clearly and succinctly to reflect their understanding.
Accurate referencing using the Harvard style is expected poor referencing will result in loss of marks.
Marks may be deducted if the assessment is not within the word limit.